Composite

Part:BBa_K2787029:Design

Designed by: Weiyi Tang   Group: iGEM18_ShanghaiTech   (2018-10-09)


RNA attenuator pT181 with GFP under pT181 target sequence by Kyoto 2013 iGEM Team


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 268
    Illegal NheI site found at 291
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1247


Design Notes

To have the sense target function properly, a RBS should be inserted between it and the GFP coding sequence. With no knowledge of the RBS sequence used by Kyoto 2013 Team, we researched and identified the RBS provided by Imperial College 2016 as workable as it functions well in their experiment of STAR RNA switch.


Source

The sense target submitted by Kyoto team was fully synthesized according to their sequence and later integrated into BBa_K2787027 through restriction cloning.


References

https://parts.igem.org/wiki/index.php?title=Part:BBa_K1126003

Westbrook, A. M. & Lucks, J. B. Achieving large dynamic range control of gene expression with a compact RNA transcription-translation regulator. Nucleic Acids Res 45, 5614-5624, doi:10.1093/nar/gkx215 (2017).